Received the B.S.degree in Chemistry from Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Airlangga, Surabaya, in 1986, the M.Sc, degree from Department of Chemistry (Biochemistry) Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, in 1994, and the Doctorate degree from Department of Biology (Microbiology), Faculty of Mathematics and Natural Sciences, Institut Pertanian Bogor, in 2004. On 1999, 2002 and 2003, she joined JSPS short course fellows in Japan. She joined post doctoral fellows program KNAW Netherlands on 2005 and 2007 at University of Groningen and also got KNAW-Multi networking Grant, The Netherlands on 2009 collaboration with Laboratory of Protein Crystallography, University of Groningen, The Netherlands.

She achieved the Professor in Biochemistry on 2010 and she was appointed as Adjunct Professor at Asia University in Taiwan in 2017-2018. From 1987 to present, she worked as Biochemistry lecturer at Dept. Of Chemistry, Faculty of Science and Technology in Universitas Airlangga. From 2011 to present, she appointed as the Head of Proteomic Laboratory at Institute of Tropical Disease in Universitas Airlangga that rebuilt as Center for Bio-Molecule Engineering in 2019. From 2007 to 2017 worked as Academic Director at Universitas Airlangga, then June 2017- December 2019, she is appointed as Excecutive Director for Airlangga Global Engagement at Universitas Airlangga. Since 2021-now she is appointed as Vice Rector for Research, Innovation and Community Development. The research grants during five years (2013 – 2019) are (1). Join Research with Institute for protein Research-Osaka University Japan on Crystallization for CBM of Geobacillus thermoleovorans IT-08 , (2). RAPID Research Grant and (3) STRANAS Grant, funding by Indonesian Ministry of Research, Technology, and Higher Education.and her current research interests include structure, mechanism and function analysis of Enzymes.

Related to the pandemic Covid19, she is appointed as Coordinator of Covid19 Research Product in Universitas Airlangga.She has already published the research at international journal, also awarded of 2 national patents. The research collaboration is being done with overseas universities, research institute, and industries. She is actively also participated as invited speaker related to International Collaboration on Higher Education strategy, output and impact. Prof. Dr. Ni Nyoman Tri Puspaningsih, M.Si is the steering committee Indonesian Society on Biochemistry and Molecular Biology (PBBMI) and Asian Community of Glycoscience and Glycotechnology (ACGG). She is also member of Indonesian Chemical Society, Indonesian Protein Society, Asean Microbiology Network and Utilization (AnMicro).